nicking reaction catalysed through the MobA89K and MobC89K proteins. The cleaved strand is then unwound with the helicase and sent to the 89K-encoded GI-style T4SS machinery. The VirB1-89K part is thought to work as a lytic transglycosylase, regionally opening the peptidoglycan with contributions from Another factor(s) in the assembly of the transp